TransTrack was developed to analyze microscopy images obtained with the SunTag translation imaging system, and can be used to acquire translation intensity traces for single mRNA molecules.
Ribofitter was developed to determine the number of translating ribosomes and the exact time at which individual ribosomes initiate translation from intensity traces obtained with translational imaging.
Most plasmids can be found on addgene.
Multi-color single-molecule imaging uncovers extensive heterogeneity in mRNA decoding
Boersma S*, Khuperkar D*, Verhagen BMP, Sonneveld S, Grimm JB, Lavis LD and Tanenbaum ME.
Cell 2019 Jul 11;178(2):458-472.e19. doi: 10.1016/j.cell.2019.05.001.
Translation and Replication Dynamics of Single RNA Viruses
Cell. 2020 Dec 23; 183, 1–16. doi: 10.1016/j.cell.2020.10.019